| DOI: 10.29090/psa.2026.04.26.10029 | Pharm Sci Asia 2026; 53(4), 534-542 |
Effect of the hydroethanolic extract of Terminalia catappa L. bark against the overproduction of cytokines TNF-α and IL-6 during neuroinflammationTREBISSOU Aïssé Florence Judith1*, KIPRE Gueyraud Rolland2, SOW Maïmouna2
1 Laboratory of Biology and Medical Research, National Institute of Public Health, Abidjan, Côte d’Ivoire
2 Laboratory of Biology and Health, UFR Biosciences, Félix Houphouët Boigny University, Abidjan, Côte d’Ivoire
The use of medicinal plants is experiencing a particularly marked resurgence of scientific interest, especially in the face of the increasing scale of neurodegenerative diseases. The general objective of our study is to evaluate in vitro the ability of Terminalia catappa L. bark to reduce the overproduction of pro-inflammatory molecules (TNF-α, IL-6) in primary rat astrocytes. The plant material consisted of dried bark of Terminalia catappa L. Ethanol extraction was performed by maceration. Four newborn rat pups (P14) were used for primary astrocyte culture. A density gradient centrifugation step using Ficoll was performed to obtain the astrocytes, which were then cultured for 14 days. Inflammation was induced using Escherichia coli lipopolysaccharide (LPS) on primary astrocyte cells. A T. catappa extract at a concentration of 50 μg/ml was chosen as the protective concentration. Ficoll density gradient separation generated three distinct fractions : astrocytes at the 0-10% interface (well-defined opalescent ring). By Day 14, a confluent monolayer (100%) of mature astrocytes had formed. Pretreatment of the cells with T. catappa extract at 50 μg/mL significantly reduced the LPS-induced increase in TNF-α from 4660 ± 0.13 pg/mL to 2685 ± 0.38 pg/mL. IL-6 decreased significantly from 5469 ± 1.58 pg/mL to 4861 ± 0.25 pg/mL. The anti-inflammatory activity of the hydroethanolic extract of Terminalia catappa L. is exceptional, with a major inhibition of TNF-α (95.6%) and IL-6 (91.5%) overproduction.
Keyword:
Neuroinflammation ; Primary astrocytes ; Terminalia catappa L. ; Côte d’Ivoir
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