| DOI: 10.29090/psa.2024.03.24.AP0835 | Pharm Sci Asia 2024; 51(3), 270-281 |
Formulations of Topical Ointment for Wound Healing Activity using Gynura procumbens (Lour.) Merr. Leaves ExtractMuntrinee Prasopsom1, Jaturong Pratuangdejkul2, Chutima Petchprayoon1, Surasak Wichaiyo3, Montree Jaturanpinyo4, Veena Satitpatipan1*
1 Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
2
Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
3
Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
4
Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Gynura procumbens (Lour.) Merr., an herb found in Southeast Asia and China, has been used in traditional medicine for treatment at a wide range of health ailments. Several biological activities were reported including wound healing activity. The previous studies revealed that ethanolic G. procumbens leaves extract contained chlorogenic acid as the major compound, which exhibited antioxidant, anti-inflammatory and wound healing potential. In this study, we aimed to develop topical formulations of G. procumbens extract and evaluate wound healing activity in mice. G. procumbens was extracted with 95% ethanol. Phytochemicals were investigated and quantified using thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) techniques. The analysis of total phenolic content (TPC), total flavonoid content (TFC) and diphenyl-picrylhydrazyl (DPPH) radical scavenging assay were performed by spectrophotometric techniques. Topical ointment of G. procumbens leaves extract for further pharmaceutical purposes was developed. The stability and shelf life of these formulations, as well as the crude extract, underwent evaluation in accordance with ASEAN guideline. Assessments were carried out at 0, 3, and 6 months under specified storage conditions. The effect of an ethanolic G. procumbens extract on wound healing activity in murine subjects was investigated. A full thickness excisional skin wound was generated on the shaved dorsum of eight-week-old C57BL/6 mice. G. procumbens ointment (0.5 or 2%w/w) or ointment base was applied once daily for 7 days. The wound size was monitored once a day for 14 days. Our results showed that ethanolic G. procumbens leaves extract contained total phenolic and total flavonoid at 43.80 ± 1.79 𝜇g GAE and 132.67 ± 1.40 𝜇g QE in 1 g extract, respectively. For DPPH radical scavenging activity, the extract exhibited IC50 of 181.70 ± 0.76 𝜇g/mL. Formulations of topical G. procumbens ointment with 0.5% and 2% w/w extract were developed. Stability study revealed that G. procumbens ointment formulas and crude extract unstable under accelerated stored at 40°C ± 2°C/75% RH ± 5% RH in tightly closed container for 6 months. All phytochemical contents decreased approximately 4–27%. For in vivo wound healing study, topical G. procumbens ointment showed a good appearance and smooth texture when applied on dorsal back of mice but did not significantly accelerated wound closure compared to the ointment base-treated controls. Therefore, although G. procumbens extract trend to potential for antioxidant and wound healing activity, it is imperative to conduct further studies on the chemical degradation pathways that could compromise the potency and quality of drug products. Additionally, employing larger sample sizes in these studies is recommended to ensure accuracy and reliability of the results.
Keyword:
Gynura procumbens; Ointment; Stability; Wound healing activity.
Download full paper (PDF File size: 660.37 KB.)
|
