| DOI: 10.29090/psa.2025.01.24.2036 | Pharm Sci Asia 2025; 52(1), 100-109 |
Enhancing Cosmetic Formulations with Pleurotus cystidiosus Extract: A Study on Anti-Photoaging and Moisturizing PotentialPariya Atawong1,2, Wannaporn Sagasae3, Jongrak Attarat3, Worasak Kaewkong3, Worawut Kriangkrai1,2,*
1 Center of Excellence for Natural Health Product Innovation and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand
2
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
3
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
Pleurotus cystidiosus, distinguished for its nutritional and biomedical attributes due to a rich repository of bioactive compounds such as antibacterial properties, antioxidant effects, and antihypertensive agents, presents promising therapeutic functionalities for cosmetic applications. This study investigated the anti-photoaging and moisturizing potential of P.cystidiosus extracts (PCE) for cosmetic application, addressing a gap in scientific exploration regarding the use of P.cystidiosus in cosmetic formulations. Through an extraction process with 60% saturation using ammonium sulfate, the results show protein yields of 72% for PCE1, 85% for PCE2, and 100% for PCE3, respectively. This demonstrates the method's efficiency with high protein yields and a rich presence of short molecular weight peptides, as confirmed by the Lowry assay and electrophoresis. These extracts demonstrated antioxidant efficacy, assessed via 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,20 -azino-bis-3- ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging assays, alongside anti-tyrosinase activity, primarily attributed to their low molecular weight fractions (<3 kDa). PCE3 demonstrated a high protein content in comparison to both PCE1 and PCE2. Therefore, PCE3 was assessed its effect on cell viability was examined through a UV exposure test. PCE3 exhibited UV radiation-protective effects against photoaging in Human keratinocytes (HaCaT) cells at 100 µg/mL concentration, suggesting its potential for sunscreen and anti-aging product integration. This study investigated the skin moisturizing effect of PCE in cosmetic formulations through snake skin moisturizing studies and water contact angle measurements, revealing an improvement in skin wettability. This enhancement in hydration highlights the critical role of PCE in formulating moisturizing products, which are integral to skincare routines.
Keyword:
Pleurotus cystidiosus; Cosmetic Applications; Anti-Photoaging; Skin Moisturizing Effect; Human Keratinocytes (HaCaT) Cells
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