| DOI: 10.29090/psa.2019.02.017.0025 | Pharm Sci Asia 2019; 46(2), 120-128 |
Simultaneous determination of salbutamol and clenbuterol
in human plasma using liquid chromatography coupled
to tandem mass spectrometryNguyen Thi Minh Thuan*,Nguyen Thi Khanh Linh
- Biochemistry Department, Pharmacy Faculty,University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Salbutamol (SAL) and clenbuterol (CLEN) are ?-agonist drugs which are illegally abused in animal feeds in an attempt to promote animal growth and to increase muscle weight. In this study, LC-MS/MS method was developed and validated to simultanously determine plasma SAL and CLEN concentrations in order to contribute to assess the toxicity and optimize the treatment efficacy of these drugs. SAL, CLEN and SAL-d3, CLEN-d9 as the internal standards were extracted from plasma samples with methanol. 10 µL of sample was injected into Inertsil® ODS-3V column C18 (150×4.6 mm, 5µm) on a HPLC system coupled to AB Sciex Triple Quad 5500 tandem mass spectrometer. The mobile phase consisted of a mixture of acetonitrile and 5mM ammonium acetate under a flow rate of 1 ml/min. The multiple reaction monitoring transitions used for identification and quantification were m/z 240→166 and m/z 240→148, respectively for SAL, and m/z 277→168 and m/z 277→203, respectively for CLEN, m/z 244→151 for SAL-d3 and m/z 286→204 for CLEN-d9, in positive ESI mode. The total run time was 15 min. The retention times for SAL and CLEN were 4.7 and 5.6 min, respectively. Linearity was in the range from 0.15-24 ppb for SAL and from 0.05-8 ppb for CLEN. Mean intra-day and inter-day precision levels for SAL and CLEN were above 90%. LLOQ of SAL and CLEN were 0.15 and 0.05 ppb, respectively. The stability study displayed SAL and CLEN degradation below 11% after 24 hours at RT. This analytical method could be used to clinical trials and assess the toxicity of these drugs.
Keyword:
Salbutamol, Clenbuterol, LC-MS/
MS, plasma
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