| DOI: 10.29090/psa.2025.02.24.3254 | Pharm Sci Asia 2025; 52(2), 230-239 |
Development of Psoriasis-Like 3D Skin Models Using the HaCaT Keratinocyte Cell line as Potential Tools in Psoriasis ResearchIntira Pathtubtim1, Celine Viennet2, Thanchanok Muangman1,*, Jarupa Viyoch3,*
1 Expert Centre of Innovative Herbal Products, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Klong Luang, Pathum Thani 12120, Thailand
2
UMR 1098 RIGHT INSERM EFS FC, DImaCell Imaging Resource Center, University of Marie&Louis Pasteur, Besan?on 25000, France
3
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand
This study focused on developing a psoriasis-like 3D skin model (RHE-Pso) utilizing HaCaT cells, which were treated with cytokine cocktails to serve as a platform for identifying anti-psoriasis agents. Reconstructed HacaT cells on high calcium condition were induced with cytokine cocktails on day 11 of culture We compared the morphology, epidermal protein, and cytokine release between the RHE-Pso and a control (RHE) that was not exposed to cytokines. The RHE-Pso model was subjected to two different cytokine formulas, which are known to be associated with psoriasis. Our findings revealed that the F1 formula (F1: 30 ng/mL TNF-α, 30 ng/mL IL-1 and 50 ng/mL IFN-γ) significantly altered the histological structure of the model more than the F2 formula (F2: 30 ng/mL IL-17 and 30 ng/mL IL-22), and resulted in lower filaggrin protein expression. Additionally, the RHE-Pso model induced with the F1 formula showed a markedly higher production of pro-inflammatory cytokines, including IL-1, IL-6, IL-8, IFN-γ, and TNF-α. Treatment of the RHE-Pso model with clobetasol propionate, an anti-psoriasis medication, effectively reduced tissue damage and decreased levels of inflammatory cytokines compared to the untreated model. This study demonstrates the potential of the RHE-Pso model as a valuable tool for exploring and validating new therapeutic compounds for psoriasis.
Keyword:
HaCaT; Psoriasis-like 3D skin model (RHE-Pso); Pro-inflammatory; Cytokine cocktails; Clobetasol propionate
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