| DOI: https://doi.org/10.14456/mujps.2016.23 | Pharm Sci Asia 2016; 43(4), 203-210 |
Cytotoxic activity of marcanine C and some other aminoquinone derivatives against MCF-7 cellsP. Thanuphol, E. Noulsri, K. Suwanborirux, N. Soonthornchareonnon, K. Pattanapanyasat*
1 Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University
2 447 Sri-Ayuthaya Rd. Rajathewi, Bangkok 10400, Thailand
3 Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University
4 Siriraj Hospital,Bangkok-noi, Bangkok 10700, Thailand
5 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University
6 254 Phayathai Rd. Bangkok 10330, Thailand
7 Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Siriraj Hospital, Bangkok-noi, Bangkok 10700, Thailand
Alkaloids from Goniothalamus have been isolated and renowned for their cytotoxicity. Recently, there has been an increment of interest to study on their chemical structures and the molecular mechanisms of action. The study herein, reported the cytotoxic effects of some Goniothalamus alkaloids in MCF-7 human breast adenocarcinoma cells as well as NB4 acute promyelocytic leukemia cells. The effects of marcanine B, C, and other structural-related compounds namely liriodenine and 5-hydroxy-3 amino-2-aceto-1,4-naphthoquinone (HAANQ) on apoptosis, caspase-3 activation and cell cycle arrest were evaluated by flow cytometry. We found that, marcanine C showed the most prominent apoptosis inducing effect on both MCF-7 and NB4 cells. As proven by the result of caspase-3 activation, marcanine C 7.5 ?M for 24 hr induced more than 23% the activation of caspase-3 on MCF-7 cells. Furthermore, marcanine C caused cell cycle arrest in the G1 phase along with low viability of the cells in S and G2/M phases suggesting the prevention of DNA synthesis and the consequent apoptosis. In summary, marcanine C exerted the most prominent cytotoxic effect among all test compounds. This study is the first to report the effects of marcanine C on apoptosis induction in breast cancer cells via caspase-3 activation and the cell cycle arrest in G1 phase.
Keyword:
marcanine C, 1-azaanthraquinones, apoptosis, cell cycle, caspase-3, Goniothalamus
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