| DOI: 10.29090/psa.2024.03.24.AP0759 | Pharm Sci Asia 2024; 51(3), 214-222 |
Establishment of an Intracranial Xenograft Model from Colorectal Cancer in Irradiated MiceTai Suc Nguyen1,2, Anh Tho Thi Tran1,2, Phuong Linh Thi Nham1, Chi Pham1, Phuong Linh Tran1, Quynh Chi Do1, Anh Vu Nguyen1, Nhu Ngoc Nguyen1,3, Mai Ly Thi Nguyen4, Przemyslaw Bozko5,6, Linh Toan Nguyen3,7, Thi Lap Nguyen2*, Khac Cuong Bui1,3,7*
1 Laboratory Animal Research Center, Vietnam Military Medical University, Hanoi, Vietnam
2
Department of Biotechnology, Faculty of Biotechnology, Hanoi University of Pharmacy, Hanoi, Vietnam
3
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam
4
Department of Biochemistry, Military Hospital 103, Hanoi, Vietnam
5
Department of Internal Medicine I, Universit?tsklinikum T?bingen, T?bingen, Germany
6
The M3 Research Institute, University of T?bingen, T?bingen, Germany
7
Vietnamese–German Centre for Medical Research (VG-CARE), Hanoi, Vietnam
Colorectal cancer (CRC) is the most common type of gastrointestinal cancer metastasizing to the brain. In addition, patients with brain metastasis from CRC have low mean survival time. Preclinical studies play a crucial role in understanding histopathological characteristics of brain tumors and the discovery of anticancer agents. To conduct preclinical studies pertaining to brain metastasis, mouse models are often based on brain-tropic cancer cell lines or spontaneous incidence in orthotropic mouse models, genetically engineered mouse models or patient-derived xenografts. These models could recapitulate metastatic processes and genetic mutations in brain metastasis, but have particular drawbacks pertaining to low yield, prolonged time and concurrent metastases in other organs. Moreover, in xenograft models, genetically immunodeficient mice are often employed because of their long-term immunodeficiency, but they still have some certain constraints. In this study, we examined the ability of the human colorectal cancer cell line HCT116 to grow into intracranial tumors in BALB/c mice immunosuppressed by irradiation. In the irradiated group, 5/5 mice had intracranial tumors with the median tumor volume reaching 4.68x106 μm3 after a 7-day follow-up. The presence of colorectal tumors in the mouse brains was confirmed by histopathology. The results showed that irradiation at the dose of 3Gy x 2 caused immunodeficiency in healthy BALB/c mice and HCT116 cells could initiate tumors intracranially in BALB/c mice immunosuppressed by irradiation with a high take rate. BALB/c mice can be used for xenograft models via immunosuppression by irradiation. In addition, the human colorectal cancer cell line HCT116 shows the potential ability to form brain tumors in research animals.
Keyword:
HCT116; Brain tumors; BALB/c mice; Irradiation
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