| DOI: https://doi.org/10.14456/mujps.2016.20 | Pharm Sci Asia 2016; 43(4), 173-182 |
Screening for the anti-angiogenic activity of selected Philippine medicinal plants using chorioallantoic membrane assayJ.E. Camposano, G.L.T. Dela Torre, J.G. Laxamana, L.L.H. Larcia II*
- College of Pharmacy, University of the Philippines – Manila, Ermita, Manila, 1000, Philippines
The search for possible cure against angiogenesis-dependent diseases is focused on plants because drugs derived naturally are inherently better tolerated in the body than synthetic drugs. Quisqualis indica, Carmona retusa and Peperomia pellucida underwent ex ovo chorioallantoic membrane (CAM) assay to determine their anti-angiogenic potential, which can provide new avenues in treating angiogenesis-dependent diseases. The crude methanolic extracts of the three plants exhibited inhibition of angiogenesis with the highest activity observed on C. retusa (49.92 + 1.53%), followed by Q. indica (29.78 + 5.93%) and P. pellucida (26.81 + 1.56%). The C. retusa crude methanolic extract was subjected to phytochemical analysis and was revealed to contain alkaloids, carbohydrates, phenols, tannins and sterols. After performing one-way ANOVA and Tukey’s post-hoc test (significant at p < 0.05), it was noted that the C. retusa crude methanolic extract has a comparable activity with the positive control, Quercetin (60.24 + 3.43%). The results suggest that C. retusa can be a potential source of anti-angiogenic compound for the design and development of drugs targeting angiogenesis-dependent diseases.
Keyword:
Carmona retusa, ex ovo chorioallantoic membrane assay, anti-angiogenic activity, quercetin
Download full paper (PDF File size: 1,154.14 KB.)
|
