| DOI: https://doi.org/10.14456/mujps.2017.2 | Pharm Sci Asia 2017; 44(1), 22-31 |
Antiproliferative effects of three roots Thai herbal recipe on human cancer cell linesU. Lueangamornnara, Y. U-pratya, W. Jiratchariyakul, A. Kulprom, P. O-charoenrat, T. Kummalue*
1 Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
2 Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
A very famous Thai herbal recipe in northeastern Thailand is named “sam rak,” which means “three roots.” These ethanolic roots extract were. The three roots Thai herbal recipe is composed of Eurycoma longifolia, Dipterocarpus obtusifolius and Tamilnadia uliginosa in a ratio of approximately 7:4:3. Ten human cancer cell lines were used in this investigation. These comprised breast (MCF-7, SKBR3, MDA-MB435), lung (A549), colon (HCT116), cervical (HeLa), leukemic (NB4 and K562), and liver (Hep3B and C3A) cancer cell lines. Human dermal fibroblasts were used as control group. ED50 values of this herbal recipe ethanolic extract were determined using MTT assay. The results showed that five cancer cell lines, HCT116, HeLa, SKBR3, NB4, and K562, were significantly inhibited with ED50 values less than 100 ?g/mL. These five cancer cell lines were further evaluated to determine their mechanisms of action using cell cycle analysis. The results demonstrated that most of these cancer cell lines showed high percentages of apoptosis, except HCT116 which showed significant S phase arrest. Thus, this study showed that “sam rak” Thai herbal recipe has potential to be developed as a traditional anticancer recipe that could be available to people all over the country.
Keyword:
Thai herbal recipe, antiproliferative effect, Dipterocarpus obtusifolius, Eurycoma longifolia, Tamilnadia uliginos
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