Simultaneous Estimation of Metaprolol Succinate and Olmesartan Medoxomil from Capsule Dosage Form by First Order Derivative Spectroscopic MethodS. D. Jadhav*, S. M. Kumbhar, V. D. Patel, R. M. Panchal and M. S. Bhatia
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Metaprolol succinate (MET) and Olmesartan medoxomil (OLM) are used in combination for treatment of hypertension. The present work deals with simple spectrophotometric method development for simultaneous estimation of MET and OLM in capsule formulation (OLSAR-M). The method employed was a first order derivative spectroscopy. For determination of sampling wavelength, 10 ?g/ml of each of MET and OLM were scanned on UV-630 double beam spectrophotometer in 200-400 nm range. The sampling wavelengths were 214 nm for OLM where MET showed zero crossing point and 231 nm for MET where OLM showed zero crossing point in first order derivative spectroscopy. For this method, linearity was observed in 10-90 ?g/ml for MET and 5-45 ?g/ml for OLM. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines. The proposed method can be optimized further for simultaneous estimation of both drugs from biological fluids, used in pharmacokinetic and bioequivalence studies.
Keyword:
Derivative Spectroscopy, Metaprolol, Olmesartan, OLSAR-M, ICH.
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